Coding

Part:BBa_K3192015

Designed by: Simonne Guenette   Group: iGEM19_Virginia   (2019-10-12)


styA

This part contains the sequence for the gene styA. styA, originating from Pseudomonas putida S12 codes for one subunit of the catabolic enzyme styrene monooxygenase. This part contains the initial enzyme subunit that begins the pathway for the metabolism of styrene to phenylacetic acid. This subunit forms a dimer with the other subunit coded by the styB gene. Together, they aid in the epoxidation of styrene into (2S)-2-phenyloxirane utilizing oxygen gas and FADH2.1

Ribbon and space-filling structure of the protein styrene monooxygenase. This is a protein dimer containing the two subunits produced by genes styA and styB. Figure obtained from Structure and Mechanism of Styrene Monooxygenase Reductase: New Insight into the FAD–Transfer Reaction.1
























References:
1) Figure 2f from: Irimia R, Gottschling M (2016) Taxonomic revision of Rochefortia Sw. (Ehretiaceae, Boraginales). Biodiversity Data Journal 4: e7720. https://doi.org/10.3897/BDJ.4.e7720. doi:10.3897/bdj.4.e7720.figure2f Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 947
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]



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