Coding
Part:BBa_K3192015
Designed by: Simonne Guenette Group: iGEM19_Virginia (2019-10-12)
styA
This part contains the sequence for the gene styA. styA, originating from Pseudomonas putida S12 codes for one subunit of the catabolic enzyme styrene monooxygenase. This part contains the initial enzyme subunit that begins the pathway for the metabolism of styrene to phenylacetic acid. This subunit forms a dimer with the other subunit coded by the styB gene. Together, they aid in the epoxidation of styrene into (2S)-2-phenyloxirane utilizing oxygen gas and FADH2.1
References:
1) Figure 2f from: Irimia R, Gottschling M (2016) Taxonomic revision of Rochefortia Sw. (Ehretiaceae, Boraginales). Biodiversity Data Journal 4: e7720. https://doi.org/10.3897/BDJ.4.e7720. doi:10.3897/bdj.4.e7720.figure2f
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 947
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 28
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |